pBAC4x-1 vector (V011668)

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LOCUS       pBAC4x-1.        5917 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with two polyhedrin and two p10 
            promoters, for the simultaneous expression of up to four proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC4x-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(1171..1219)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    1222..1248
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1251..1360)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1493..1503
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1511..1602)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1627..1736
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1744..1755
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    1756..1804
                     /label=poly(A) signal
                     /note="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        1842..1933
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    1937..1966
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1967..1984
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     2021..3378
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3641..4229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4403..5260)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5261..5365)
                     /label=AmpR promoter
     rep_origin      5392..5847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"