pBACgus-1 vector (V011667)

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LOCUS       pBACgus-1.        7408 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the polyhedrin promoter, encoding 
            beta-glucuronidase plus an optional C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    3412..3457
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3458..3475
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     3512..4869
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5132..5720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5894..6751)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6752..6856)
                     /label=AmpR promoter
     rep_origin      6883..7338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"