pBACgus4x-1 vector (V011662)

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LOCUS       pBACgus4x-1.        8066 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid encoding beta-glucuronidase, with two 
            polyhedrin and two p10 promoters, for the simultaneous expression of
            up to four proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus4x-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     polyA_signal    complement(3320..3368)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3371..3397
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3400..3509)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3642..3652
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3660..3751)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3776..3885
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3893..3904
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    3905..3953
                     /label=poly(A) signal
                     /note="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        3991..4082
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4086..4115
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             4116..4133
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     4170..5527
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5790..6378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6552..7409)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7410..7514)
                     /label=AmpR promoter
     rep_origin      7541..7996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"