Basic Vector Information
- Vector Name:
- pBiEx-2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6162 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
- Promoter:
- IE1
pBiEx-2 vector Map
pBiEx-2 vector Sequence
LOCUS pBiEx-2. 6162 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for high-level expression in both bacterial and insect cells of proteins with a cleavable N-terminal GST-6xHis-S-Tag cassette plus a C-terminal HSV tag. ACCESSION . VERSION . KEYWORDS pBiEx-2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6162) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 6162) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6162 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..483 /label=hr5 enhancer /note="baculovirus early transcription enhancer" promoter 487..1078 /label=IE1 promoter /note="promoter of the ie1 gene from the baculovirus Autographa californica" promoter 1092..1110 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1111..1135 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1150..1172 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 1185..1835 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 1872..1889 /label=6xHis /note="6xHis affinity tag" CDS 1899..1943 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS 1959..1976 /label=thrombin site /note="thrombin recognition and cleavage site" CDS 1995..2009 /label=enterokinase site /note="enterokinase recognition and cleavage site" misc_feature 2008..2115 /label=MCS /note="MCS" /note="multiple cloning site" CDS 2121..2153 /label=HSV tag /note="HSV (herpes simplex virus) epitope tag" terminator 2223..2270 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2295..2602 /label=IE1 terminator /note="terminator of the ie1 gene from the baculovirus Autographa californica" protein_bind complement(2690..2711) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(2727..3806) /label=lacI /note="lac repressor" promoter complement(3807..3884) /label=lacI promoter promoter 4016..4120 /label=AmpR promoter CDS 4121..4978 /label=AmpR /note="beta-lactamase" rep_origin 5152..5740 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 6028..6049 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 6064..6094 /label=lac promoter /note="promoter for the E. coli lac operon" primer_bind 6126..6142 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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