pBiEx-2 vector (V011657)

Download GeneBank File(.gb)

LOCUS       pBiEx-2.        6162 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in both bacterial and insect cells 
            of proteins with a cleavable N-terminal GST-6xHis-S-Tag cassette 
            plus a C-terminal HSV tag.
ACCESSION   .
VERSION     .
KEYWORDS    pBiEx-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        1092..1110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1111..1135
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1150..1172
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1185..1835
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             1872..1889
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1899..1943
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1959..1976
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1995..2009
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    2008..2115
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2121..2153
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     terminator      2223..2270
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2295..2602
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     protein_bind    complement(2690..2711)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2727..3806)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3807..3884)
                     /label=lacI promoter
     promoter        4016..4120
                     /label=AmpR promoter
     CDS             4121..4978
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5152..5740
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6028..6049
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6064..6094
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     6126..6142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"