pBiEx-3 vector (V011656)

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LOCUS       pBiEx-3.        5373 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in both bacterial and insect cells 
            of proteins with a C-terminal S-Tag-8xHis cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBiEx-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        1092..1110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1111..1135
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1150..1172
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1177..1273
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1179..1181
                     /codon_start=1
                     /label=ATG
                     /note="ATG"
                     /translation="M"
     CDS             1284..1328
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1350..1373
                     /label=8xHis
                     /note="8xHis affinity tag"
     terminator      1434..1481
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1506..1813
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     protein_bind    complement(1901..1922)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1938..3017)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3018..3095)
                     /label=lacI promoter
     promoter        3227..3331
                     /label=AmpR promoter
     CDS             3332..4189
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4363..4951
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5239..5260
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5275..5305
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     5337..5353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"