pDream2.1/MCS vector (V011650)

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LOCUS       pDream2.1_MCS.        7159 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression of N-terminally FLAG-tagged proteins in 
            bacterial, insect, and mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDream2.1 MCS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   GenScript
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1810..1812
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1813..1836
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1837..1882
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     protein_bind    complement(1905..1929)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1931..2407
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     terminator      2408..2455
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2548..2976
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2990..3319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3386..4177
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4354..4475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4519..4574
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_recomb     4722..5427
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     protein_bind    complement(5436..5469)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(5637..6224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6240..7097)
                     /label=AmpR
                     /note="beta-lactamase"