Basic Vector Information
- Vector Name:
- pIB/V5-His
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3521 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- OpIE-2
pIB/V5-His vector Map
pIB/V5-His vector Sequence
LOCUS pIB_V5-His. 3521 bp DNA circular SYN 01-JAN-1980
DEFINITION Insect cell vector for transient or stable constitutive expression
of C-terminally V5-6xHis-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pIB V5-His
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3521)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3521)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3521
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..548
/label=OpIE-2 promoter
/note="strong constitutive baculovirus promoter for insect
cell expression"
misc_feature 557..652
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 659..700
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 710..727
/label=6xHis
/note="6xHis affinity tag"
polyA_signal 745..874
/label=OpIE-2 poly(A) signal
/note="baculovirus polyadenylation signal"
rep_origin complement(1002..1590)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 1665..1956
/label=OpIE-1 promoter
/note="moderate constitutive baculovirus promoter for
insect cell expression"
promoter 1982..2029
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2048..2443
/label=BSD
/note="blasticidin S deaminase"
CDS 2566..3423
/label=AmpR
/note="beta-lactamase"
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