pIDC vector (V011642)

Basic Vector Information

Vector Name:
pIDC
Antibiotic Resistance:
Chloramphenicol
Length:
2312 bp
Type:
Insect Cell Vectors
Replication origin:
R6K γ ori
Source/Author:
Geneva Biotech
Copy Number:
High copy number
Promoter:
polyhedrin

pIDC vector Map

pIDC2312 bp60012001800loxPR6K gamma oriCmRcat promoterSV40 poly(A) signalMCSpolyhedrin promoter

pIDC vector Sequence

LOCUS       pIDC.        2312 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector with a polyhedrin promoter and a 
            chloramphenicol resistance marker. Serves as a donor vector in the 
            MultiBac(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    30..63
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      84..472
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(867..1523)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1524..1626)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     polyA_signal    complement(1798..1932)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2069..2154
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2191..2282)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"

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