Basic Vector Information
- Vector Name:
- pIDC
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 2312 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- R6K γ ori
- Source/Author:
- Geneva Biotech
- Copy Number:
- High copy number
- Promoter:
- polyhedrin
pIDC vector Vector Map
pIDC vector Sequence
LOCUS pIDC. 2312 bp DNA circular SYN 01-JAN-1980
DEFINITION Baculovirus transfer vector with a polyhedrin promoter and a
chloramphenicol resistance marker. Serves as a donor vector in the
MultiBac(TM) system.
ACCESSION .
VERSION .
KEYWORDS pIDC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2312)
AUTHORS Geneva Biotech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 2312)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..2312
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 30..63
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
rep_origin 84..472
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(867..1523)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(1524..1626)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
polyA_signal complement(1798..1932)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 2069..2154
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter complement(2191..2282)
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
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