pIDK vector (V011641)

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LOCUS       pIDK.        2281 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector with a p10 promoter and a kanamycin 
            resistance marker. Serves as a donor vector in the MultiBac(TM) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..124
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    132..195
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    260..308
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    510..543
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      545..901
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             1262..2053
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"