pIEx-5 vector (V011624)

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LOCUS       pIEx-5.        3843 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of secreted 
            proteins with a C-terminal S-Tag-8xHis cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     sig_peptide     1084..1143
                     /label=AKH signal sequence
                     /note="signal sequence from tobacco hornworm adipokinetic 
                     hormone"
     misc_feature    1145..1241
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1252..1296
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1318..1341
                     /label=8xHis
                     /note="8xHis affinity tag"
     terminator      1360..1667
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1697..1801
                     /label=AmpR promoter
     CDS             1802..2659
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2833..3421
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3709..3730
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3745..3775
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3783..3799
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"