pIEx-6 vector (V011623)

Download GeneBank File(.gb)

LOCUS       pIEx-6.        3783 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of proteins with a 
            cleavable N-terminal 6xHis tag and a C-terminal S-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     CDS             1084..1086
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1090..1107
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1126..1140
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1219..1263
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      1300..1607
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1637..1741
                     /label=AmpR promoter
     CDS             1742..2599
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2773..3361
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3649..3670
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3685..3715
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3723..3739
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3747..3763
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"