pIZ/V5-His vector (V011614)

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LOCUS       pIZ_V5-His.        2876 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact insect cell vector with a Zeocin(TM) resistance marker, for 
            constitutive expression of proteins with an optional C-terminal 
            V5-6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIZ V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..552
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    561..656
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             663..704
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             714..731
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    749..878
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1666..2213
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=immediate-early gene 2 from the Orgyia pseudots
                     /note="OpIE-2 promoter"
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     promoter        2242..2289
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2309..2680
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2747..2876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"