pIZT/V5-His vector (V011613)

LOCUS       pIZT_V5-His.        3336 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector with a GFP-Zeocin(TM) resistance fusion gene, for
            constitutive expression of proteins with an optional C-terminal 
            V5-6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIZT V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..552
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    567..656
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             663..704
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             714..731
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    749..878
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1669..1960
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        1986..2033
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2052..2054
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2067..2771
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     CDS             2766..3143
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=Sh ble from Streptoalloteichus hindustanus
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
                     REFALRDPAGNCVHFVAEEQD"
     polyA_signal    3207..3336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"