pMIB/V5-His B vector (V011608)

LOCUS       pMIB_V5-His_B.        3600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            A or pMIB/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     sig_peptide     565..627
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    629..731
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             738..779
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             789..806
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    824..953
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1081..1669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1744..2035
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2061..2108
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2127..2522
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2645..3502
                     /label=AmpR
                     /note="beta-lactamase"