pMT/V5-His B vector (V011601)

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LOCUS       pMT_V5-His_B.        3542 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for inducible high-level expression of C-terminally 
            V5-6xHis-tagged proteins in Drosophila cells. For other reading 
            frames, use pMT/V5-His A or pMT/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMT V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..3542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        407..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     misc_feature    849..933
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             934..975
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             985..1002
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(1044..1178)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1322..1338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1346..1362)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1370..1400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1415..1436)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1724..2312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2486..3343)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3344..3448)
                     /label=AmpR promoter