pQE-TriSystem His-Strep1 vector (V011598)

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LOCUS       pQE-TriSystem_Hi        5760 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of 
            proteins with C-terminal Strep-Tag II and 8xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem His-Strep1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     misc_feature    1265..1372
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1267..1269
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1381..1404
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1411..1434
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1435..1445
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1589..1644
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1732..1779
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1792..3250
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3409..3996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4012..4869)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4935..5760
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"