pQE-TriSystem His-Strep2 vector (V011597)

Download GeneBank File(.gb)

LOCUS       pQE-TriSystem_Hi        5796 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of 
            proteins with an N-terminal Strep-Tag II tag and a C-terminal 8xHis 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem His-Strep2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     CDS             1267..1269
                     /codon_start=1
                     /label=ATG
                     /note="ATG"
                     /note="start codon"
                     /translation="M"
     CDS             1279..1302
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1408..1440
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             1447..1470
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1471..1481
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1625..1680
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1768..1815
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1828..3286
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3445..4032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4048..4905)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4971..5796
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"