pGL2-Control vector (V011547)

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LOCUS       pGL2-Control.        6047 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector with the SV40 enhancer and promoter driving strong 
            luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Control
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        42..238
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             268..1917
                     /label=luciferase
                     /note="firefly luciferase"
     intron          2161..2226
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2356..2376
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2801..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2947..3176
                     /note="SV40 enhancer"
     rep_origin      complement(3505..4093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4267..5124)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5125..5229)
                     /label=AmpR promoter
     rep_origin      5256..5711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5901..6022
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"