pGL2-Enhancer vector (V011546)

LOCUS       pGL2-Enhancer.        5855 bp DNA     circular SYN 01-JAN-1980
DEFINITION  SV40 enhancer-containing vector for measuring the activity of 
            promoter sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Enhancer
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..52
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             76..1725
                     /label=luciferase
                     /note="firefly luciferase"
     intron          1969..2034
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2164..2184
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2609..2743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2755..2984
                     /note="SV40 enhancer"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4075..4932)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4933..5037)
                     /label=AmpR promoter
     rep_origin      5064..5519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5709..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"