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pGL3-Control vector (V011543)

Price Information

Cat No. Plasmid Name Availability Add to cart
V011543 pGL3-Control In stock, 1 week for quality controls

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Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
pGL3-Control
Antibiotic Resistance:
Ampicillin
Length:
5256 bp
Type:
Luciferase Vectors
Replication origin:
ori
Source/Author:
Promega
Copy Number:
High copy number

pGL3-Control vector Map

Copy Sequence View Map
pGL3-Control5256 bp6001200180024003000360042004800ColE1-derived plasmid replication originmultiple cloning siteSV40 promoterluciferaseSV40 poly(A) signalregulatoryRV primer4 sequencing primer binding siteoriAmpRAmpR promoterf1 oripoly(A) signalpause site

Plasmid Protocol

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5. Store the plasmid at -20 ℃.

6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it

General Plasmid Transform Protocol

1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.

2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.

3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.

4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.

5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.

pGL3-Control vector Sequence

LOCUS       40924_21958        5256 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGL3-Control, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Groskreutz DJ, Schenborn ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1996) D.J. Groskreutz, R
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 5256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-1996) D.J. Groskreutz, R"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Road, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Mar 5, 2001 this sequence version replaced U47296.1.
FEATURES             Location/Qualifiers
     source          1..5256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..41
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             280..1929
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1973..2094)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2205..2441
                     /regulatory_class="enhancer"
     primer_bind     complement(2499..2518)
                     /label=RV primer4 sequencing primer binding site
                     /note="RV primer4 sequencing primer binding site"
     rep_origin      2756
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(2759..3347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3521..4378)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4379..4483)
                     /label=AmpR promoter
     rep_origin      4510..4965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5096..5144
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5158..5249
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"