pGL4.16[luc2CP/Hygro] vector (V011534)

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LOCUS       pGL4.16[luc2CP_H        6020 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding highly destabilized luciferase for 
            measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.16[luc2CP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1756..1803
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
     CDS             1807..1926
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1975..2096)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2290..2647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2678..3712
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3739..3787
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4114..4702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4905..5762)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5867..5915
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5929..6020
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"