pGL4.20[luc2/Puro] vector (V011530)

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LOCUS       pGL4.20[luc2_Pur        5404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.20[luc2 Puro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1796..1917)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2111..2468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2499..3095
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3123..3171
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3498..4086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4289..5146)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5251..5299
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5313..5404
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"