pGL4.29[luc2P/CRE/Hygro] vector (V011521)

Download GeneBank File(.gb)

LOCUS       pGL4.29[luc2P_CR        6084 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a cAMP response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.29[luc2P CRE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    44..109
                     /label=bind
                     /bound_moiety="cAMP response element-binding protein CREB"
                     /note="CRE"
                     /note="cyclic AMP response element (3 copies)"
     promoter        151..182
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             215..1864
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1868..1987
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2039..2160)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2354..2711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2742..3776
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3803..3851
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4178..4766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4969..5826)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5931..5979
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5993..6084
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"