pGL4.34[luc2P/SRF-RE/Hygro] vector (V011516)

LOCUS       pGL4.34[luc2P_SR        6112 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a serum response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.34[luc2P SRF-RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..143
                     /label=serum response factor (SRF) binding site
                     /bound_moiety="serum response factor (SRF)"
                     /note="SRF-RE"
                     /note="serum response factor response element (5 copies)"
     promoter        179..210
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             243..1892
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1896..2015
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2067..2188)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2382..2739
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2770..3804
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3831..3879
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4206..4794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4997..5854)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5959..6007
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6021..6112
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"