pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (V011515)

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LOCUS       pGL4.35[luc2P_9X        6184 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a promoter and an extended Gal4 upstream activating 
            sequence for studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.35[luc2P 9XGAL4UAS Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..130
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        222..262
                     /note="Ad promoter"
                     /note="adenovirus major late promoter"
     CDS             315..1964
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1968..2087
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2139..2260)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2454..2811
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2842..3876
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3903..3951
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5069..5926)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    6031..6079
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6093..6184
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"