pGL4.36[luc2P/MMTV/Hygro] vector (V011514)

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LOCUS       pGL4.36[luc2P_MM        7246 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with an MMTV LTR for studying cell signaling using 
            destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.36[luc2P MMTV Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..1328
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     CDS             1377..3026
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             3030..3149
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(3201..3322)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3516..3873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3904..4938
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4965..5013
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5340..5928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6131..6988)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    7093..7141
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    7155..7246
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"