pGL4.47[luc2P/SIE/Hygro] vector (V011504)

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LOCUS       pGL4.47[luc2P_SI        6129 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a sis-inducible element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.47[luc2P SIE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..409
                     /label=b
                     /bound_moiety="STAT3 homodimers and STAT3/STAT1
                     heterodimers"
                     /note="SIE"
                     /note="sis-inducible element (5 copies)"
     promoter        454..485
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             518..2167
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2171..2290
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2342..2463)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2657..3014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3045..4079
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4106..4154
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4481..5069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5272..6129)
                     /label=AmpR
                     /note="beta-lactamase"