pGL4.84[hRlucCP/Puro] vector (V011484)

Download GeneBank File(.gb)

LOCUS       pGL4.84[hRlucCP        4866 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding highly destabilized Renilla luciferase 
            for measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.84[hRlucCP Puro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1032
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1039..1086
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
     CDS             1090..1209
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1258..1379)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1573..1930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1961..2557
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    2585..2633
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2960..3548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3751..4608)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4713..4761
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4775..4866
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"