pMCS-Cypridina Luc vector (V011471)

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LOCUS       pMCS-Cypridina_L        5989 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Secreted Cypridina luciferase vector for cloning a transcriptional 
            regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Cypridina Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..1707
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1733..1844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1967..2296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2344..2391
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2410..3006
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3139..3272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3316..4173)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4437..5025
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5295..5311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5767..5783
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5898..5989
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"