pMCS-Red Firefly Luc vector (V011467)

LOCUS       pMCS-Red_Firefly        5977 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Intracellular red firefly luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Red Firefly Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..1692
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     polyA_signal    1721..1832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1955..2284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2332..2379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2398..2994
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3127..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3304..4161)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4425..5013
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5283..5299
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5755..5771
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5886..5977
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"