pNL1.3.CMV[secNluc/CMV] vector (V011456)

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LOCUS       pNL1.3.CMV[secNl        3945 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Co-transfection vector for expressing secreted NanoLuc(R) luciferase
            under control of the strong CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pNL1.3.CMV[secNluc CMV]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             859..945
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             946..1455
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(1499..1620)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2039..2627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2830..3687)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3792..3840
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3854..3945
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"