pNL1.3[secNluc] vector (V011455)

MSDS

Basic Vector Information

Vector Name:: pNL1.3[secNluc]

Antibiotic Resistance:: Ampicillin

Length:: 3194 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Promega

Copy Number:: High copy number

pNL1.3[secNluc] vector Vector Map

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pNL1.3[secNluc] vector Sequence

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LOCUS       pNL1.3[secNluc].        3194 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL1.3[secNluc]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..186
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             187..696
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(748..869)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1288..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2079..2936)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3041..3089
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3103..3194
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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