pNL2.3[secNluc/Hygro] vector (V011452)

MSDS

Basic Vector Information

Vector Name:: pNL2.3[secNluc/Hygro]

Antibiotic Resistance:: Ampicillin

Length:: 4793 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Promega

Copy Number:: High copy number

Promoter:: SV40

pNL2.3[secNluc/Hygro] vector Vector Map

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pNL2.3[secNluc/Hygro] vector Sequence

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LOCUS       pNL2.3[secNluc_H        4793 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL2.3[secNluc Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             187..696
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(748..869)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1063..1420
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1451..2485
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2512..2560
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2887..3475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3678..4535)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4640..4688
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4702..4793
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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