pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] vector (V011450)

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LOCUS       pNL3.2.NF-kB-RE[        4912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and NF-kappa-B response element for 
            studying cell signaling using destabilized NanoLuc(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..84
                     /label=NF-kappa-B binding site
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B RE"
                     /note="NF-kappa-B response element (5 copies)"
     promoter        116..147
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             180..692
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             696..815
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(867..988)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1182..1539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1570..2604
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2631..2679
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3006..3594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3797..4654)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4759..4807
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"