Basic Vector Information
- Vector Name:
- pNL3.2[NlucP/minP]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3274 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Copy Number:
- High copy number
- Promoter:
- minP
pNL3.2[NlucP/minP] vector Vector Map
pNL3.2[NlucP/minP] vector Sequence
LOCUS pNL3.2[NlucP_min 3274 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using destabilized NanoLuc(R)
luciferase.
ACCESSION .
VERSION .
KEYWORDS pNL3.2[NlucP minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3274)
AUTHORS Promega
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3274)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3274
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..70
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/label=minP
/note="minimal TATA-box promoter with low basal activity"
CDS 141..653
/label=Nluc
/note="NanoLuc(R) luciferase"
CDS 657..776
/label=hPEST
/note="PEST degradation sequence from mouse ornithine
decarboxylase"
polyA_signal complement(828..949)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1368..1956)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2159..3016)
/label=AmpR
/note="beta-lactamase"
polyA_signal 3121..3169
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3183..3274
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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