pNL3.3[secNluc/minP] vector (V011448)

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LOCUS       pNL3.3[secNluc_m        3235 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using secreted NanoLuc(R) 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.3[secNluc minP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..227
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             228..737
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(789..910)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1329..1917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2120..2977)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3082..3130
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3144..3235
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"