pSELECT-zeo-Lucia vector (V011443)

LOCUS       pSELECT-zeo-Luci        3769 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for the expression of secreted Lucia(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSELECT-zeo-Lucia
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   InvivoGen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    9..57
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    68..159
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        196..407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             420..688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             728..1357
                     /codon_start=1
                     /product="secreted coelenterazine-utilizing luciferase"
                     /label=secreted coelenterazine-utilizing luciferase
                     /note="Lucia(R)"
                     /note="synthetic gene based on luciferases from marine
                     copepods"
                     /translation="MEIKVLFALICIAVAEAKPTEINEDLNIAAVASNFATTDLETDLF
                     TNWETMNVISTDTEQVNTDADRGKLPGKKLPPDVLRELEANARRAGCTRGCLICLSHIK
                     CTPKMKKFIPGRCHTYEGEKESAQGGIGEAIVDIPEIPGFKDKEPLDQFIAQVDLCADC
                     TTGCLKGLANVQCSDLLKKWLPQRCTTFASKIQGRVDKIKGLAGDR"
     polyA_signal    complement(1379..1500)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1600..1994)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2011..2382)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(2402..2449)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(2495..2698)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2699..3002)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"