pSP-luc+NF vector (V011417)

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LOCUS       40924_40997        4103 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSP-luc+NF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Brondyk WH, Groskreutz DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) R
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4103)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-1996) R"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2000) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Road, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On May 9, 2000 this sequence version replaced U47123.1.
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1
                     /label=SP6 RNA polymerase initiation site
                     /note="SP6 RNA polymerase initiation site"
     misc_feature    11..53
                     /label=upstream multiple cloning site
                     /note="upstream multiple cloning site"
     CDS             54..1697
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="DAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHI
                     EVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPAN
                     DIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMY
                     TFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARD
                     PIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQ
                     SALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGL
                     TETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGY
                     VNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQ
                     HPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFV
                     DEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    1702..1731
                     /label=downstream multiple cloning site
                     /note="downstream multiple cloning site"
     promoter        complement(1741..1759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2014
                     /label=ColE1-derived replication origin
                     /note="ColE1-derived replication origin"
     rep_origin      complement(2017..2605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2779..3636)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3637..3741)
                     /label=AmpR promoter
     promoter        4087..4103
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"