pSV40-GLuc vector (V011415)

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LOCUS       pSV40-GLuc.        4178 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector with a strong constitutive SV40 promoter for 
            expressing secreted Gaussia luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pSV40-GLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..45
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        51..246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             306..860
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     polyA_signal    complement(898..1019)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1681..2269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2443..3300)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3301..3405)
                     /label=AmpR promoter
     rep_origin      3432..3887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4018..4066
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4080..4171
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"