pTK-CLuc vector (V011414)

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LOCUS       pTK-CLuc.        7000 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector with a constitutive HSV thymidine kinase promoter 
            for expressing secreted Cypridina luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pTK-CLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..770
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             801..2459
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     misc_feature    2463..2494
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(2510..2631)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2866..3294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3308..3638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3705..4496
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4673..4806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4843..4859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4867..4883)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4891..4921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4936..4957)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5245..5833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6007..6864)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6865..6969)
                     /label=AmpR promoter