pXPG vector (V011407)

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LOCUS       40924_47258        6087 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXPG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN.
  TITLE     Generation of an improved luciferase reporter gene plasmid that 
            employs a novel mechanism for high-copy replication
  JOURNAL   Plasmid 44 (2), 173-182 (2000)
  PUBMED    10964627
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2000) Division of Human Immunology, Hanson Centre 
            For Cancer Research, Institute of Medical and Veterinary Science, 
            Frome Road, Adelaide, SA 5000, Australia
REFERENCE   3  (bases 1 to 6087)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2000) Division of Human Immunology, Hanson Centre 
            For Cancer Research, Institute of Medical and Veterinary Science, 
            Frome Road, Adelaide, SA 5000, Australia
REFERENCE   4  (bases 1 to 6087)
  AUTHORS   Cockerill PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2004) Molecular Medicine, University of Leeds, 
            Clinical Sciences Building, St. James's University Hospital, Leeds 
            LS9 7TF, UK
REFERENCE   5  (bases 1 to 6087)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2000"; volume: "44"; issue: "2"; pages: "173-182"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2000) Division of Human Immunology, Hanson Centre For Cancer
            Research, Institute of Medical and Veterinary Science, Frome Road, 
            Adelaide, SA 5000, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2000) Division of Human Immunology, Hanson Centre For Cancer
            Research, Institute of Medical and Veterinary Science, Frome Road, 
            Adelaide, SA 5000, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2004) Molecular Medicine, University of Leeds, Clinical 
            Sciences Building, St. James's University Hospital, Leeds LS9 7TF, 
            UK"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Mar 25, 2004 this sequence version replaced AF262031.2.
            The reporter gene plasmid pXPG has been engineered by incorporating 
            a novel high copy origin of replication and the modified Luc+ 
            luciferase gene into the pXP1 vector.
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..46
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             76..1725
                     /label=luciferase
                     /note="firefly luciferase"
     intron          1813..1878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2008..2028
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2450..2584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3441..3545
                     /label=AmpR promoter
     CDS             3546..4403
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4577..5165
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5168
     misc_feature    complement(5351..5491)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      5633..5862
                     /label=SV40 early polyA signal
                     /note="SV40 early polyA signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    5730..5864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      5863..6087
                     /label=SV40 early polyA signal
                     /note="SV40 early polyA signal"
                     /regulatory_class="polyA_signal_sequence"