pcDNA4/His A vector (V011311)

LOCUS       pcDNA4_His_A.        5097 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for expressing proteins with a cleavable N-terminal
            6xHis tag. For other reading frames, use pcDNA(TM)4/His B or 
            pcDNA(TM)4/His C.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA4 His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..922
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             932..949
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             953..985
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             989..1012
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    1012..1085
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1111..1335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1381..1809
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1823..2152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2200..2247
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2266..2637
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2770..2903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2940..2956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2964..2980)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2988..3018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3033..3054)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3342..3930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4104..4961)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4962..5066)
                     /label=AmpR promoter