pcDNA4/HisMax A vector (V011308)

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LOCUS       pcDNA4_HisMax_A.        5258 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for high-level expression of proteins with a 
            cleavable N-terminal 6xHis tag. For other reading frames, use 
            pcDNA(TM)4/HisMax B or pcDNA(TM)4/HisMax C.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA4 HisMax A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    917..1079
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5'
                     UTR of the mouse VEGF gene"
     CDS             1080..1082
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1092..1109
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1113..1145
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             1149..1172
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    1172..1245
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1271..1495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1541..1969
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1983..2313
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2361..2408
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2427..2798
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2931..3064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3101..3117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3125..3141)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3149..3179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3194..3215)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3503..4091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4265..5122)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5123..5227)
                     /label=AmpR promoter