Basic Vector Information
- Vector Name:
- pcDNA4/V5-His A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5092 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- EM7
pcDNA4/V5-His A vector Vector Map
pcDNA4/V5-His A vector Sequence
LOCUS pcDNA4_V5-His_A. 5092 bp DNA circular SYN 01-JAN-1980 DEFINITION Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA(TM)4/V5-His B or pcDNA(TM)4/V5-His C. ACCESSION . VERSION . KEYWORDS pcDNA4 V5-His A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5092) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 5092) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT The cloned gene must include a Kozak sequence and start codon. FEATURES Location/Qualifiers source 1..5092 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 895..1008 /label=MCS /note="MCS" /note="multiple cloning site" CDS 1009..1050 /label=V5 tag /note="epitope tag from simian virus 5" CDS 1060..1077 /label=6xHis /note="6xHis affinity tag" polyA_signal 1106..1330 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1376..1804 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1818..2147 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 2195..2242 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2261..2632 /label=BleoR /note="antibiotic-binding protein" polyA_signal 2765..2898 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2935..2951) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2959..2975) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2983..3013) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3028..3049) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3337..3925) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4099..4956) /label=AmpR /note="beta-lactamase" promoter complement(4957..5061) /label=AmpR promoter
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