pcDNA4/V5-His B vector (V011297)

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LOCUS       pcDNA4_V5-His_B.        5096 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for expressing C-terminally V5- and 6xHis-tagged 
            proteins. For other reading frames, use pcDNA(TM)4/V5-His A or 
            pcDNA(TM)4/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA4 V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..1012
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1013..1054
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1064..1081
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1110..1334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1380..1808
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1822..2151
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2199..2246
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2265..2636
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2769..2902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2939..2955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2963..2979)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2987..3017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3032..3053)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3341..3929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4103..4960)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4961..5065)
                     /label=AmpR promoter