pCMV-HA vector (V011249)

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LOCUS       pCMV-HA.        3782 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing an N-terminally HA-tagged protein in mammalian
            cells. Identical to pCMV-HA-N.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             829..831
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             832..858
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    863..917
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(921..1055)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1166..1182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1190..1206)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1214..1244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1259..1280)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1568..2156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2330..3187)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3188..3292)
                     /label=AmpR promoter
     primer_bind     3766..3782
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"