Basic Vector Information
The pCMV-MIR vector is designed for the expression of microRNA (miRNA) precursors under the control of the CMV promoter. It includes a GFP reporter for monitoring transfection efficiency and a neomycin resistance gene for selection purposes.
- Vector Name:
- pCMV-MIR
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6219 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- OriGene
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- SV40
- Growth Strain(s):
- DH10b
- Growth Temperature:
- 37℃
pCMV-MIR vector Map
References
- McGrath J, Kane LE, Maher SG. The Influence of MicroRNA-31 on Oxidative Stress and Radiosensitivity in Pancreatic Ductal Adenocarcinoma. Cells. 2022;11(15):2294. Published 2022 Jul 25. doi:10.3390/cells11152294
pCMV-MIR vector Sequence
LOCUS 40924_11700 6219 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for expressing microRNA (miRNA) precursors using the CMV
promoter, with a GFP reporter for transfection and a neomycin
resistance marker.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6219)
AUTHORS OriGene
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6219)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Inserts are cloned between the SgfI (AsiSI) and MluI sites.
FEATURES Location/Qualifiers
source 1..6219
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 166..182
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 343..722
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 723..926
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 952..970
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1084..1113
/codon_start=1
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
CDS 1132..1155
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
primer_bind complement(1175..1191)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 1202..1824
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
rep_origin complement(1973..2561)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal complement(2890..2937)
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(3162..3857)
/codon_start=1
/label=TurboGFP
/note="improved green fluorescent protein from Pontellina
plumata (Evdokimov et al., 2006)"
/translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
KTPDADAGEE"
misc_feature complement(3858..4437)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS complement(4462..5253)
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(5288..5645)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter complement(5647..5751)
/label=AmpR promoter
rep_origin 5778..6206
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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