pCMV-Myc vector (V011245)

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LOCUS       pCMV-Myc.        3791 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing an N-terminally Myc-tagged protein in 
            mammalian cells. pCMV-Myc-N is identical but contains a complete Myc
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid contains a truncated Myc tag.
FEATURES             Location/Qualifiers
     source          1..3791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             829..831
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             841..867
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="Myc (truncated)"
                     /note="The first amino acid of the standard Myc tag is
                     absent."
                     /translation="QKLISEEDL"
     misc_feature    872..926
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(930..1064)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1175..1191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1199..1215)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1223..1253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1268..1289)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1577..2165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2339..3196)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3197..3301)
                     /label=AmpR promoter
     primer_bind     3775..3791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"