pCMV6-XL5-DDK-His vector (V011171)

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LOCUS       pCMV6-XL5-DDK-Hi        4626 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian transient expression vector for 
            fusing an ORF to C-terminal FLAG(R) and 6xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-XL5-DDK-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    979..1072
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1079..1102
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1109..1126
                     /label=6xHis
                     /note="6xHis affinity tag"
     primer_bind     complement(1179..1195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1206..1828
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1857..2186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2434..3022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3196..4053)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4054..4158)
                     /label=AmpR promoter
     rep_origin      4185..4613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"