pEF1α-Tet3G vector (V011159)

MSDS

Basic Vector Information

Vector Name:: pEF1α-Tet3G

Antibiotic Resistance:: Ampicillin

Length:: 7859 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Source/Author:: Clontech

Selection Marker:: Neomycin/G418(Geneticin)

Copy Number:: High copy number

Promoter:: EF-1α

pEF1α-Tet3G vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEF1α-Tet3G vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pEF1alpha-Tet3G.        7859 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the Tet-On(R) 3G transactivator protein, which 
            allows for inducible gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    pEF1alpha-Tet3G.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        206..1387
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1495..2238
                     /label=Tet-On(R) 3G
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of 
                     doxycycline"
     polyA_signal    2251..2385
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3105..3693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3867..4724)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4725..4829)
                     /label=AmpR promoter
     polyA_signal    complement(4942..5076)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5501..5521)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(5651..5716)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(6139..6930)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7291..7620)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"

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